TOOLS 5X EvaGreen qPCR Mix without ROX
TOOLS 5X EvaGreen qPCR Mix without ROX is optimized for real-time quantitative PCR assays. The ready-to-load mix includes TOOLS HotTaq DNA polymerase, ultrapure dNTPs, MgCl2, EvaGreen® dye. Only water, template and primers needs to be added. TOOLS HotTaq DNA polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
- 產品特點
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Fast qPCR results and high performance.
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Minimal inhibition of PCR ensures maximum deficiency, sensitivity, and reproducibility.
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Robust discrimination and reproducibility.
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Unique "release-on-demand" DNA binding mechanism of EvaGreen dye enables superior PCR and melt curve analysis results.
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Novel chemically-modified hotstart Taq, requiring only 15 minutes to activate.
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Compatible with both fast and regular cycling protocols.
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EvaGreen dye in the master mix can also serve asa gel stain - Analyze your PCR product by gel electrophoresis without the need to add another gel stain.
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